Life Science

O-PTIR imaging and spectroscopy of Bacteria

Simultaneous, sub-micron IR (O-PTIR) and Raman spectroscopy and imaging of individual bacterial cells

Single bacterial cell O-PTIR microscopy with deuterium labelled E. coli

A: O-PTIR image at 1655cm^-1 (protein) at 200nm step size.

B: O-PTIR image at 2195cm^-1 (C-D stretch) at 200nm step size. Both images took 3 min to acquire each.

C: Single E. Coli cell (2.6×1.3 microns) imaged at 1655cm^-1 with 50nm steps. Image time, ~1 min.

D: Four submicron (~500nm spot) O-PTIR spectra were acquired from the single bacterial cell image above (Upper Right), with corresponding colors. Spectra are normalized to 1655cm^-1.

Each spectrum is 10 averages (~15 secs). You can see the C-D absorbances at around 2195cm^-1 and 2100cm^-1.

Single bacterial cell simultaneous submicron IR+Raman Microscopy

Upper Left: Visible image of bacterial cells. Orange box indicate region of IR imaging.

Bottom Left: O-PTIR infrared image at 1655cm^-1, with 50nm step size.

Collection time ~1 min.

Right: Simultaneous, submicron IR and Raman spectra collected from the indicated spot on the single bacterial cell in the IR image. Spectra are normalised to the most intense band spectra and are ~20sec accumulations. Raman spectra are baseline corrected.

Here, the clear complementarities of IR and Raman spectroscopy are evident, with the strong C-H stretching bands in the Raman (~2900cm^-1) being characteristically strong, whilst the IR spectra are very strong with their protein (~1600cm^-1) and other fingerprint range (1800-800cm^-1) signatures

The inherent sensitivity advantage of IR is also clear, with the O-PTIR spectrum demonstrating many times better sensitivity (SNR) over the Raman spectrum despite both measurements being the same time, ~20sec.

Webinars

Life Science

Alzheimer’s Disease Research with Sub-micron Simultaneous IR+Raman: Co-localization of Beta-Sheets and Carotenoids in Aggregates

Two-part webinar to learn how submicron IR (O-PTIR) and Raman has been applied to characterize the co-localization of amyloid β aggregates with carotenoids in human brain tissues to help better understand Alzheimer’s Disease progression.

Life Science

Life science applications using novel submicron simultaneous IR and Raman microscopy – A new paradigm in vibrational spectroscopy

Professor Ji-Xin Cheng of Boston University Photonics Center, author of over 230 peer-reviewed papers, co-inventor of CARS microscopy and pioneer in the field of O-PTIR applied to life science systems, in conjunction with Dr Mustafa Kansiz, Director of Product Management at PSC are proud to present this webinar.

Life Science

Amyloid aggregates in neurons – Life science applications using submicron simultaneous IR and Raman microscopy

Dr Oxana Klementieva and co-workers identifying amyloid-beta aggregates directly in neurons (neurites an dendritic spines) at the subcellular (submicron) level using Optical Photothermal Infrared (O-PTIR) imaging (mIRage submicron Infrared Microscope).

Life Science

Collagen orientation, fiber to submicron fibril life science applications of O-PTIR, from tissues to single cells and bacteria

In this webinar, we will show how, submicron simultaneous Optical-Photothermal IR (O-PTIR) spectroscopy and Raman microscopy (IR+Raman) is being used and published in life science applications, from tissues, to cells and even single bacterial cells.

Life Science

Single and intra-cell bacterial IR spectroscopy

Life science applications of O-PTIR, from tissues to live cells and single cell bacteria. In this webinar, we will show how submicron simultaneous Optical-Photothermal IR (O-PTIR) spectroscopy and Raman microscopy (IR+Raman) is being used and published in life science applications, from tissues, to cells and even single bacterial cells.

Life Science

Live single cell analysis with simultaneous submicron IR+Raman spectroscopy

In this webinar, Prof Peter Gardner (Manchester University, UK) will present results from their recent and first published study on live (and fixed) cell analysis with simultaneous submicron IR+Raman microscopy with wide spectral coverage using two pump lasers (QCL and OPO).

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