Simultaneous sub-micron O-PTIR and Raman Spectroscopy
The world’s first simultaneous IR+Raman microscopy system is a unique dual modality platform with all the advantages of an O-PTIR and Raman microscope combined. O-PTIR, optical photothermal microscopy, using a visible probe laser, is combined with Raman for applications in polymers, defect analysis, microplastic detection and red blood cells.
Spectra, line scans and 2D maps can now be collected from the same spot at the same time, opening up new research opportunities and a more thorough characterization of your sample.
All the features and capabilities of the mIRage platform
Simultaneous sub-micron O-PTIR and Raman spectroscopy, same spot, same time, same resolution
High quality IR and Raman spectra with combined database for both Raman and IR
for complementary and confirmatory materials ID
Wide range of applications
miRage-R provides unique data for numerous material types, including:
- Defect identification
- Forensics
- Geological sciences
- Polymers & Composites
- Pharmaceutical
- Micro-electronics
- Life Science
- Cultural heritage
Simultaneous IR and Raman spectral searching, complementary and confirmatory
Traditionally IR and Raman spectral searching has been done separately and the data has been analyzed separately. Now, with Wiley’s recent release of the KnowItAll™ multi-technique of simultaneous searching, the hit list information is displayed in a graphical 2D scatter plot. This multi-dimensional approach plots the HQI of the unknown IR spectrum against the HQI of the unknown Raman spectrum dramatically speeding up data analysis. This greatly simplified display of spectral matches in graphical rather than tabular form greatly improves the accuracy of the identification. IR search results can confirm Raman search results and vice-versa, all simultaneously.
Sub-micron O-PTIR + Raman of microplastics
mIRage locates PS (0.9 µm, 2.0 µm, 4.5 µm and 10 µm) and PMMA beads (3.0 µm) in salt crystal mixture in hi-res IR images at key absorption bands. Distortion free spectra, even amongst salt crystals at hotspots, confirm the identity of the microplastics and readily searched against IR database. Importantly, and unlike traditional FTIR/QCL systems, spectra are consistent, regardless of particle shape or size when measured in reflection mode – no dispersive scatter artefacts.
Single bacterial cell simultaneous submicron IR+Raman microscopy
A: Visible image of bacterial cells. Orange box indicate region of IR imaging. B: O-PTIR infrared image at 1655cm-1, with 50nm step size. Collection time ~1 min. C: Simultaneous, submicron IR and Raman spectra collected from the indicated spot on the single bacterial cell. Spectra are normalized to the most intense band spectra are ~20sec accumulations. O-PTIR spectra are collected with a Dual Range (C-H/FP) QCL, covering 3000-2700, 1800-950cm-1 in a single unit. O-PTIR spectra are raw (no processing). Raman spectra are baseline corrected.
SNR of the OPTIR (~500nm spot) is ~4000:1 (RMS, taking amide band intensity as the peak and the baseline noise at the amide I position measured on a CaF2 blank) with ~20 sec accumulations.
IR+Raman analysis of red blood cells
The mIRage-R provides unparalleled productive measurements red blood cells
Left: Optical image with selected 70 x 70 µm area for subsequent Raman imaging
Right: IR+Raman spectra collected off of a selected red blood cell (~500 nm resolution).
Data sheet:
Beyond the limits of traditional
IR microscopy. IR+Raman: Same time. Same spot. Same resolution.
Application note:
Sub-micron IR and Raman spectroscopy for chemical identification of microplastics
Fluorescence guided sub-micron IR and simultaneous Raman spectroscopy: A world first and only
Widefield fluorescence detected sub-500nm infrared multimodal microscopy
mIRage Spectroscopy options
Counter-propagating mode and other mIRage spectroscopy capabilities to extend research flexibility.
Multi-modal Imaging options
Complimentary O-PTIR techniques such as simultaneous Raman and co-located Fluorescence.
mIRage Environmental options
mIRage environmental options broaden the applications range with heating, cooling and flow cells.
Need more information?
Discover how O-PTIR technology can elevate your research or help solve your toughest challenges. Our team are happy to assist and answer your questions.
