Life Science

Hard tissue

High resolution IR and Raman Imaging of hard tissue matter

Sub-micron IR analysis of tooth lateral cross-section

Despite rough topography, dispersion-artefact free, spatially resolved submicron IR spectra and IR chemical images showing the chemical difference between the enamel vs. the dentin could be obtained in reflection mode off thick slices of teeth.
Protein absorption are more prominent in the dentin while the enamel has stronger phosphate absorption from hydroxyapatite

IR Polarized O-PTIR to study collagen orientation in individual fibrils and tendon

A: Spectra obtained with O-PTIR from control tendon fibrils on CaF2 window. B: Single frequency image at right recorded at 1655 cm^-1 in perpendicular orientation. markers denote locations at which spectra were acquired. Scale bar = 1µm

C and D: Optical photothermal IR (O-PTIR) spectra from intact tendon, from ~500 nm measurement spots. (B) Individual spectra obtained from the two orientations of a section mounted on a CaF2 window, relative to the linearly polarized QCL. Inserted visual image shows the 6 locations, all of which lie within the region imaged with FTIR FPA; scale bar = 70 μm.

Colored markers (+) correspond to spectral colors. (C) Comparison of spectra obtained from CaF2 (top) and glass (bottom) substrates in parallel and perpendicular orientations to linearly polarized QCL.

Published: Gorker Bakir et al., “Orientation Matters: Polarization Dependent IR Spectroscopy of Collagen from Intact Tendon Down to the Single Fibril Level”, Molecules 2020, 25, 4295

Widefield O-PTIR measurements on autofluorescent collagen fibril

FL-PTIR measurements on an autofluorescent collagen fibril at different orientations. (A) Autofluorescent emission image of collagen fibril excited at 365 nm. (B-C) FL-PTIR absorption images at 1660 cm^-1, 1550 cm^-1, respectively. (D) FL-PTIR spectra extracted from an IR hyperspectral image stack from the indicated regions of interest for IR radiation oriented substantially perpendicular (E) to the fibril.

(Sample courtesy of Prof. K Gough, University of Manitoba).

Webinars

Life Science

Alzheimer’s Disease Research with Sub-micron Simultaneous IR+Raman: Co-localization of Beta-Sheets and Carotenoids in Aggregates

Two-part webinar to learn how submicron IR (O-PTIR) and Raman has been applied to characterize the co-localization of amyloid β aggregates with carotenoids in human brain tissues to help better understand Alzheimer’s Disease progression.

Life Science

Life science applications using novel submicron simultaneous IR and Raman microscopy – A new paradigm in vibrational spectroscopy

Professor Ji-Xin Cheng of Boston University Photonics Center, author of over 230 peer-reviewed papers, co-inventor of CARS microscopy and pioneer in the field of O-PTIR applied to life science systems, in conjunction with Dr Mustafa Kansiz, Director of Product Management at PSC are proud to present this webinar.

Life Science

Amyloid aggregates in neurons – Life science applications using submicron simultaneous IR and Raman microscopy

Dr Oxana Klementieva and co-workers identifying amyloid-beta aggregates directly in neurons (neurites an dendritic spines) at the subcellular (submicron) level using Optical Photothermal Infrared (O-PTIR) imaging (mIRage submicron Infrared Microscope).

Life Science

Collagen orientation, fiber to submicron fibril life science applications of O-PTIR, from tissues to single cells and bacteria

In this webinar, we will show how, submicron simultaneous Optical-Photothermal IR (O-PTIR) spectroscopy and Raman microscopy (IR+Raman) is being used and published in life science applications, from tissues, to cells and even single bacterial cells.

Life Science

Single and intra-cell bacterial IR spectroscopy

Life science applications of O-PTIR, from tissues to live cells and single cell bacteria. In this webinar, we will show how submicron simultaneous Optical-Photothermal IR (O-PTIR) spectroscopy and Raman microscopy (IR+Raman) is being used and published in life science applications, from tissues, to cells and even single bacterial cells.

Life Science

Live single cell analysis with simultaneous submicron IR+Raman spectroscopy

In this webinar, Prof Peter Gardner (Manchester University, UK) will present results from their recent and first published study on live (and fixed) cell analysis with simultaneous submicron IR+Raman microscopy with wide spectral coverage using two pump lasers (QCL and OPO).

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