Please join Assoc. Prof. Ayanjeet Ghosh from the Dept of Chemistry and Biochemistry, Uni of Alabama, AL, USA, in this two-part webinar to learn how submicron IR (O-PTIR) and Raman has been applied to characterize the co-localization of amyloid β aggregates with carotenoids in human brain tissues to help better understand Alzheimer’s Disease progression. Results will be presented from their recent publication.
Taking full advantage of the complementarity of IR and Raman (now fully exploitable with sub-micron O-PTIR having the same spatial resolution as Raman), protein secondary structure was assessed (where IR spectroscopy excels) together with Raman, which through a resonance Raman effect with 532nm excitation, enhances the carotenoid signals. Together, these two techniques have revealed for the first time a clear and strong association of carotenoids with β sheet protein structures.
Additionally, Dr Mustafa Kansiz, Director of Product Management at Photothermal Spectroscopy Corp, will provide a brief technique overview, covering submicron simultaneous IR+Raman with co-located fluorescence widefield imaging for improved optical contrast to guide the measurement the process.
Webinar highlights;
Part I:
- Overview of submicron O-PTIR and simultaneous Raman with co-located fluorescence imaging
- Comparisons and advantages over current traditional FTIR and Raman microscopy techniques
- A review of applications, drawing on a variety of recent high impact publication examples across a range of life science examples
Part II:
- O-PTIR unequivocally demonstrates the presence of elevated β-sheets in carotenoid containing plaques and the lack of β structure in noncarotenoid plaques.
- Demonstration of how submicron IR is naturally complimentary to Raman, offering better β sheet protein confirmation detection with Raman (through Resonance) delivering better carotenoid signals.
