In this webinar, we will show how, submicron simultaneous Optical-Photothermal IR (O-PTIR) spectroscopy and Raman microscopy (IR+Raman) is being used and published in life science applications, from tissues, to cells and even single bacterial cells. Prof Kathy Gough (Uni Manitoba, Canada) will present results from her recent publication on collagen, tendon and fibril analysis with O-PTIR, where polarized IR light was used to gain insight into hierarchical molecular orientation. Spectra and images were obtained from sections of intact oriented tendon (on CaF2 and glass) and individual fibrils of ~500 nm diameter, to yield the first confirmatory and complementary polarized data on a biopolymer. The Amide I and II bands in fibril spectra were narrower than those from the intact tendon, whilst both relative intensities and band shapes were altered. These spectra represent reliable profiles for normal collagen type I fibrils under polarized IR light, and can serve as a benchmark for the study of collagenous tissues.
In conjunction with Dr Mustafa Kansiz, Director of Product Management at PSC we are proud to present this cutting-edge webinar.
The webinar will be recorded for later on-demand viewing with opportunities to ask questions during and after the webinar.
