Join Dr Yeran Bai, Dept of Molecular, Cellular and Developmental Biology, Uni California Santa Barbara, in this two-part webinar to learn how submicron IR (O-PTIR) spectroscopy and imaging is being applied for the study of lipid metabolic heterogeneity in single cells, using novel IR tags, based on azide conjugated fatty acid precursors. Vibrational spectroscopy imaging integrated with vibrational (IR) probes provides a new direction for single-cell metabolic analysis. Vibrational probes are biorthogonal, enabling selective detection of metabolic products without interference from cellular endogenous molecules, such as bulky fluorescent labels traditional used in fluorescence imaging.
Dr Bai will present a single cell metabolic imaging platform to enable direct imaging of lipid metabolism, detecting newly synthesized lipids, with high specificity in various human-derived 2D and 3D culture systems. Furthermore, the platform provides for simultaneous fluorescence imaging for cell-type identification. Additionally, Dr Mustafa Kansiz, Director of Product Management at Photothermal Spectroscopy Corp, will provide a brief technique overview, covering submicron simultaneous IR+Raman with co-located fluorescence widefield imaging, with advances now delivering sub-500nm IR spatial resolution.
This two-part event will present these breakthroughs with a range of application examples, from life sciences to particulates/microplastics, failure analysis, materials sciences, and more.
